Recovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA.

Abstract : Detailed studies of host/parasite interactions are currently limited because in situ gene sequencing or monitoring of parasite gene expression is so far limited to genes presenting a high loci copy number in the Schistosome genome or a high level of expression. Indeed, how to investigate the host parasite molecular interplay when parasites are not directly accessible in vivo? Here we describe a method to circumvent this problem and to analyze DNA and RNA of Schistosoma mansoni during the interaction with its intermediate snail host Biomphalaria glabrata. We propose a technique for improved DNA and RNA extraction from the intra-molluscan stage of the parasite recovered after fixation of infected snails in Raillet-Henry solution. The extractions can be used for genetic analysis, transcription studies and microsatellite genotyping.
Document type :
Journal articles
Complete list of metadatas

Cited literature [32 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/halsde-00612896
Contributor : Anne Modat <>
Submitted on : Thursday, February 2, 2012 - 4:15:06 PM
Last modification on : Thursday, February 7, 2019 - 2:54:06 PM
Long-term archiving on : Tuesday, December 13, 2016 - 6:07:04 PM

File

Tapuscrit-Allienne-2011-ExpPar...
Files produced by the author(s)

Identifiers

Citation

Jean-François Allienne, André Théron, Benjamin Gourbal. Recovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA.. Experimental Parasitology, Elsevier, 2011, 129 (1), pp.11-16. ⟨10.1016/j.exppara.2011.06.003⟩. ⟨halsde-00612896⟩

Share

Metrics

Record views

351

Files downloads

282