Involvement of the cytokine MIF in the snail host immune response to the parasite Schistosoma mansoni.

Abstract : We have identified and characterized a Macrophage Migration Inhibitory Factor (MIF) family member in the Lophotrochozoan invertebrate, Biomphalaria glabrata, the snail intermediate host of the human blood fluke Schistosoma mansoni. In mammals, MIF is a widely expressed pleiotropic cytokine with potent pro-inflammatory properties that controls cell functions such as gene expression, proliferation or apoptosis. Here we show that the MIF protein from B. glabrata (BgMIF) is expressed in circulating immune defense cells (hemocytes) of the snail as well as in the B. glabrata embryonic (Bge) cell line that has hemocyte-like features. Recombinant BgMIF (rBgMIF) induced cell proliferation and inhibited NO-dependent p53-mediated apoptosis in Bge cells. Moreover, knock-down of BgMIF expression in Bge cells interfered with the in vitro encapsulation of S. mansoni sporocysts. Furthermore, the in vivo knock-down of BgMIF prevented the changes in circulating hemocyte populations that occur in response to an infection by S. mansoni miracidia and led to a significant increase in the parasite burden of the snails. These results provide the first functional evidence that a MIF ortholog is involved in an invertebrate immune response towards a parasitic infection and highlight the importance of cytokines in invertebrate-parasite interactions.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/halsde-00608628
Contributeur : Anne Modat <>
Soumis le : mercredi 13 juillet 2011 - 15:50:21
Dernière modification le : mercredi 5 juin 2019 - 17:32:18
Document(s) archivé(s) le : vendredi 14 octobre 2011 - 02:41:36

Fichier

Garcia-2010-PlosPathog-Involve...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Alvaro Baeza Garcia, Raymond Pierce, Benjamin Gourbal, Elisabeth Werkmeister, Dominique Colinet, et al.. Involvement of the cytokine MIF in the snail host immune response to the parasite Schistosoma mansoni.. PLoS Pathogens, Public Library of Science, 2010, 6 (9), pp.e1001115. ⟨10.1371/journal.ppat.1001115⟩. ⟨halsde-00608628⟩

Partager

Métriques

Consultations de la notice

683

Téléchargements de fichiers

782