ZAP-70 intron 1 DNA methylation status: Determination by pyrosequencing in B chronic lymphocytic leukemia

Abstract : ZAP-70 expression is a strong prognostic indicator in chronic lymphocytic leukemia. However, ZAP-70 quantification by flow cytometry lacks sufficient standardization. Based upon the correlation between ZAP-70 expression and its gene methylation status, we have developed a quantitative pyrosequencing assay for the determination of ZAP-70 methylation adapted for routine use. Methylation in four CpG pairs (C-223, C-243, C-254, and C-267) in the first intron of ZAP-70 is associated with repression of ZAP-70. Moreover, it correlates with CD38 expression (n = 111, p < .0001), IgHv mutation status (n = 106, p < .0001), time to treatment (p < .0001), and overall survival (p = .0014). Pyrosequencing of ZAP-70 provides a good alternative to flow cytometry.
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https://hal.archives-ouvertes.fr/halsde-00495188
Contributeur : Anne Modat <>
Soumis le : vendredi 25 juin 2010 - 11:34:04
Dernière modification le : jeudi 25 juillet 2019 - 14:24:03

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S.P. Chantepie, S. Vaur, Christoph Grunau, V. Salaün, M. Briand, et al.. ZAP-70 intron 1 DNA methylation status: Determination by pyrosequencing in B chronic lymphocytic leukemia. Leukemia Research, Elsevier, 2010, 34 (6), pp.800-808. ⟨10.1016/j.leukres.2009.10.018⟩. ⟨halsde-00495188⟩

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