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Conference papers
Environmental DNA metabarcoding to investigate historic changes in biodiversity
Johan Pansu
1
Charline Giguet-Covex
2
Francesco Ficetola Gentile
1
Ludovic Gielly
1
Frederic Boyer
1
Eric Coissac
1
Isabelle Domaizon
3
Lucie Zinger
4
Jérôme Poulenard
2
Fabien Arnaud
2
Johan Pansu 1
Author
Charline Giguet-Covex 2
Author IdHAL : charline-giguet-covex
Francesco Ficetola Gentile 1
Author
Ludovic Gielly 1
Author
Frederic Boyer 1
Author
Eric Coissac 1
Author
Isabelle Domaizon 3
Author Employer institution : Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement IdHAL : isabelle-domaizon ORCID : https://orcid.org/0000-0001-9785-3082
Lucie Zinger 4
Author
Jérôme Poulenard 2
Author IdHAL : jeromepoulenarduniv-savoiefr ORCID : https://orcid.org/0000-0003-0810-0308
Fabien Arnaud 2
Author IdHAL : fabien-arnaud ORCID : https://orcid.org/0000-0002-8706-9902 ResearcherId : http://www.researcherid.com/rid/F-7003-2012
1
LECA - Laboratoire d'Ecologie Alpine (bat. D - Biologie 2233 Rue de la piscine - BP 53 38041 GRENOBLE CEDEX 9 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5553 (France)
- USMB [Université de Savoie] [Université de Chambéry] - Université Savoie Mont Blanc (27, rue Marcoz - 73000 Chambéry - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
2
EDYTEM - Environnements, Dynamiques et Territoires de la Montagne (Université de Savoie, Campus scientifique, 73376 Le Bourget du Lac cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5204 / FRE 2641 (France)
- USMB [Université de Savoie] [Université de Chambéry] - Université Savoie Mont Blanc (27, rue Marcoz - 73000 Chambéry - France)
3
CARRTEL - Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (74200 Thonon-les-Bains, France - 73376 Le Bourget du Lac, France
- France)
- INRA - Institut National de la Recherche Agronomique : UMR0042 (France)
- USMB [Université de Savoie] [Université de Chambéry] - Université Savoie Mont Blanc (27, rue Marcoz - 73000 Chambéry - France)
4
EDB - Evolution et Diversité Biologique (Université Toulouse III Paul Sabatier
Bâtiment 4R1
118, route de Narbonne
31062 Toulouse cedex 9
France - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- IRD - Institut de Recherche pour le Développement (Siège Le Sextant 44, bd de Dunkerque CS 90009 13572 Marseille cedex 02 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5174 (France)
Abstract : Paleoenvironmental studies provides crucial long-term data to study both anthropogenic disturbance and/or climate changes, and their effects on the environment. We used a multi-disciplinary approach to reconstruct past landscapes in Lake Anterne watershed (Haute-Savoie, French Alps) over the Holocene. In a previous study, DNA metabarcoding approach was carried out to evidence the important role of livestock farming on erosion processes (Giguet-Covex et al. submitted). Here, we focus on impact of climate changes and human activities on plant cover. DNA extracted from lacustrine archives was amplified with universal primers for plants and sequenced using high-throughput sequencing. For each period, we selected ecologically informative taxa (i.e. taxa specific of particular conditions : plant community, soils, land-use…) in the aim to obtain an image of the environment at this time. These results combined with previously obtained geochemical and sedimentological approaches allowed to reconstruct past landscapes and to determine drivers of changes.
Document type :
Conference papers
Complete list of metadata
https://hal-sde.archives-ouvertes.fr/hal-01879611
Contributor : Christine Maury Referent Hal Edytem <>
Submitted on : Monday, September 24, 2018 - 10:31:17 AM
Last modification on : Wednesday, September 8, 2021 - 1:52:23 PM
Contributor : Christine Maury Referent Hal Edytem <>
Submitted on : Monday, September 24, 2018 - 10:31:17 AM
Last modification on : Wednesday, September 8, 2021 - 1:52:23 PM
Identifiers
- HAL Id : hal-01879611, version 1
Collections
CNRS | INRA | LECA | UNIV-SAVOIE | UNIV-TLSE3 | INRAE | UGA | CARRTEL | EDYTEM
Citation
Johan Pansu, Charline Giguet-Covex, Francesco Ficetola Gentile, Ludovic Gielly, Frederic Boyer, et al.. Environmental DNA metabarcoding to investigate historic changes in biodiversity. 6th. Congrès International « Barcode of Life », 2015, guelph, Canada. p. 264. ⟨hal-01879611⟩
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