, H3K27me3 and H4K20me1 between genotypes Fa and Fb in sympatric condition. The track Ref, p.717

S. , mansoni displays transcripts (exons as blocks and introns as arrows) deduced 718 from RNA-Seq data in cercariae

, ) a mollusk intermediate host (Biomphalaria genus) in which it multiplies asexually as 722 sporocysts and then cercariae, and (2) a vertebrate definitive host (primate or rodent) in which 723 adult worms reproduce sexually. Two types of free-swimming larvae, miracidia and cercariae, are 724 the infective form for the mollusk and vertebrate hosts, respectively. Eggs laid by adult worm 725 couples in the mesenteric vein of the vertebrate host can go through the intestine wall to be 726

, using the same amount of S. mansoni chromatin 729 (equivalent to 20 adult worms) and an increasing amount of antibody. Percentage of input 730 recovery of chromatin was measured by qPCR on two housekeeping genes, alpha tubulin and 28S, 731 as described in (Cosseau et al. 2009). Titrations were done to measure the efficiency of 732 immunoprecipitation for antibody H3K27ac Abcam cat# ab4729 lot#, p.733

, H4K20me1 Abcam, cat# ab9051 lot# GR158874-1 (B and C)

, colors") as identified by EpiCSeg in 736 adults. (A) Composition in histone marks of the five chromatin states. Color code of the states is 737 used in all other parts of this figure. (B) Proportion of the genome covered by each state in adults

, Enrichment for each state around gene bodies (C), at transcription start site (TSS) (D) and 739 transcription end site (TES) (E) of adult worms

, Supplementary Figure 4: Principal Component Analysis (PCA) for the four studied histone marks in 743 cercariae (A) and adults (B). Only sites with statistically significant differences (épimutations) were

, Blue circles (Bre) represent cercariae and adults from sympatric conditions, p.745

, Cercariae sample GuaFb5 for H3K4me3 746 was excluded from analysis (see methods)

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