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Journal articles
Copper homeostasis at the host vibrio interface: lessons from intracellular vibrio transcriptomics
A.S. Vanhove
1
T.P. Rubio
1
A.N. Nguyen
2, 3
A. Lemire
4
D. Roche
5
J. Nicod
1
Agnès Vergnes
1
Aurore Poirier
1
E. Disconzi
2
Evelyne Bachère
1
F. Le Roux
4
Annick Jacq
2, 3
Guillaume M Charrière
1
Delphine Destoumieux-Garzón
1
Guillaume M Charrière 1
Author
Delphine Destoumieux-Garzón 1
Mail :
Author IdHAL : ddestoum ORCID : https://orcid.org/0000-0002-8793-9138
1
IHPE - Interactions Hôtes-Pathogènes-Environnements (Université de Perpignan Via Domitia Bâtiment R 52 Avenue Paul Alduy 66860 Perpignan cedex (Languedoc-Roussillon) - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5244 (France)
- UM - Université de Montpellier (163 rue Auguste Broussonnet - 34090 Montpellier - France)
- IFREMER - Institut Français de Recherche pour l'Exploitation de la Mer (155, rue Jean-Jacques Rousseau - 92 138 Issy-les-Moulineaux Cedex - France)
- UPVD - Université de Perpignan Via Domitia (52 avenue Paul Alduy - 66860 Perpignan Cedex 9 - France)
2
I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
3
SRRB - Signalisation et Réseaux de Régulations Bactériens (I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- DBG - Département Biologie des Génomes (I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
- I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
4
LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins (Station Biologique de Roscoff Place Georges Teissier 29680 Roscoff - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8227 (France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- SBR - Station biologique de Roscoff [Roscoff] (Place Georges Teissier - BP 74 29682 ROSCOFF CEDEX - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : FR2424 (France)
5
UMR 8030 - Génomique métabolique (GENOSCOPE, 2 rue Gaston Crémieux 91057 Evry Cedex - France)
- GENOSCOPE - Genoscope - Centre national de séquençage [Evry] : DRF/JACOB (2, rue Gaston Crémieux CP5706 91057 EVRY Cedex - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/JACOB (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8030 (France)
- UEVE - Université d'Évry-Val-d'Essonne (Boulevard François Mitterrand 91025 Evry Cedex - France)
Abstract : Recent studies revealed that several vibrio species have evolved the capacity to survive inside host cells. However, it is still often ignored if intracellular stages are required for pathogenicity. Virulence of Vibrio tasmaniensis LGP32, a strain pathogenic for Crassostrea gigas oysters, depends on entry into hemocytes, the oyster immune cells. We investigated here the mechanisms of LGP32 intracellular survival and their consequences on the host-pathogen interaction. Entry and survival inside hemocytes were required for LGP32-driven cytolysis of hemocytes, both in vivo and in vitro. LGP32 intracellular stages showed a profound boost in metabolic activity and a major transcription of antioxidant and copper detoxification genes, as revealed by RNA sequencing. LGP32 isogenic mutants showed that resistance to oxidative stress and copper efflux are two main functions required for vibrio intracellular stages and cytotoxicity to hemocytes. Copper efflux was also essential for host colonization and virulence in vivo. Altogether our results identify copper resistance as a major mechanism to resist killing by phagocytes, induce cytolysis of immune cells and colonize oysters. Selection of such resistance traits could arise from vibrio interactions with copper-rich environmental niches including marine invertebrates, which favor the emergence of pathogenic vibrios resistant to intraphagosomal killing across animal species.
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https://hal-sde.archives-ouvertes.fr/hal-01311061
Contributor : Anne Modat <>
Submitted on : Tuesday, May 3, 2016 - 3:59:32 PM
Last modification on : Thursday, March 12, 2020 - 4:56:01 PM
Contributor : Anne Modat <>
Submitted on : Tuesday, May 3, 2016 - 3:59:32 PM
Last modification on : Thursday, March 12, 2020 - 4:56:01 PM
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Identifiers
- HAL Id : hal-01311061, version 1
- DOI : 10.1111/1462-2920.13083
Collections
CNRS | UNIV-EVRY | UPMC | LBI2M | CEA | UNIV-PERP | CEA-UPSAY | I2BC | GENOMIQUE-METABOLIQUE | UNIV-PARIS-SACLAY | B3ESTE | UNIV-MONTPELLIER | IHPE | JACOB | CEA-DRF | JOLIOT | SORBONNE-UNIVERSITE | SU-SCIENCES | FR2424 | GENOSCOPE
Citation
A.S. Vanhove, T.P. Rubio, A.N. Nguyen, A. Lemire, D. Roche, et al.. Copper homeostasis at the host vibrio interface: lessons from intracellular vibrio transcriptomics. Environmental Microbiology, Wiley-Blackwell, 2016, 18 (3), pp.875-888. ⟨10.1111/1462-2920.13083⟩. ⟨hal-01311061⟩
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