Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1fish liver cell line.

Aude Kienzler (1) , Barbara J. Mahler (2) , Peter C. van Metre (2) , Nathalie Schweigert (1) , Alain Devaux (1) , Sylvie Bony (1)

1 LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés
2 US Geological Survey [Austin]

Coal-tar-based (CTB) sealcoat, frequently applied to parking lots and driveways in North America, contains elevated concentrations of polycyclic aromatic hydrocarbons (PAHs) and related compounds. The RTL-W1 ␣sh liver cell line was used to investigate two endpoints (genotoxicity and DNA-repair-capacity impairment) associated with expo- sure to runoff from asphalt pavement with CTB sealcoat or with an asphalt-based sealcoat hypothesized to contain about 7% CTB sealcoat (AS-blend). Genotoxic potential was assessed by the Formamido pyrimidine glycosylase (Fpg)-modi␣ed comet assay for 1:10 and 1:100 dilutions of runoff samples collected from 5 h to 36 d following sealcoat application. DNA-repair capacity was assessed by the base excision repair comet assay for 1:10 dilution of samples collected 26 h and 36 d following application. Both assays were run with and without co-exposure to ultraviolet-A radiation (UVA). With co-exposure to UVA, genotoxic effects were signi␣cant for both dilutions of CTB runoff for three of four sample times, and for some samples of AS-blend runoff. Base excision repair was signif- icantly impaired for CTB runoff both with and without UVA exposure, and for AS-blend runoff only in the absence of UVA. This study is the ␣rst to investigate the effects of exposure to the complex mixture of chemicals in coal tar on DNA repair capacity. The results indicate that co-exposure to runoff from CT-sealcoated pavement and UVA as much as a month after sealcoat application has the potential to cause genotoxicity and impair DNA repair capacity.

Keywords: Genotoxicity; DNA repair activity; Polycyclic aromatic hydrocarbon; N-heterocycle; Coal tar; Urban runoff

Domains: Biodiversity and Ecology

Aude Kienzler, Barbara J. Mahler, Peter C. van Metre, Nathalie Schweigert, Alain Devaux, et al.. Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1fish liver cell line.. Science of the Total Environment, 2015, 520, pp.73-80. ⟨10.1016/j.scitotenv.2015.03.005⟩. ⟨hal-01292436⟩