Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1fish liver cell line.

Aude Kienzler; Barbara J. Mahler; Peter C. van Metre; Nathalie Schweigert; Alain Devaux; Sylvie Bony

Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1fish liver cell line.

Aude Kienzler ¹ Barbara J. Mahler ² Peter C. van Metre ² Nathalie Schweigert ¹ Alain Devaux ¹ Sylvie Bony ¹

1 LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés

Abstract : Coal-tar-based (CTB) sealcoat, frequently applied to parking lots and driveways in North America, contains elevated concentrations of polycyclic aromatic hydrocarbons (PAHs) and related compounds. The RTL-W1 ␣sh liver cell line was used to investigate two endpoints (genotoxicity and DNA-repair-capacity impairment) associated with expo- sure to runoff from asphalt pavement with CTB sealcoat or with an asphalt-based sealcoat hypothesized to contain about 7% CTB sealcoat (AS-blend). Genotoxic potential was assessed by the Formamido pyrimidine glycosylase (Fpg)-modi␣ed comet assay for 1:10 and 1:100 dilutions of runoff samples collected from 5 h to 36 d following sealcoat application. DNA-repair capacity was assessed by the base excision repair comet assay for 1:10 dilution of samples collected 26 h and 36 d following application. Both assays were run with and without co-exposure to ultraviolet-A radiation (UVA). With co-exposure to UVA, genotoxic effects were signi␣cant for both dilutions of CTB runoff for three of four sample times, and for some samples of AS-blend runoff. Base excision repair was signif- icantly impaired for CTB runoff both with and without UVA exposure, and for AS-blend runoff only in the absence of UVA. This study is the ␣rst to investigate the effects of exposure to the complex mixture of chemicals in coal tar on DNA repair capacity. The results indicate that co-exposure to runoff from CT-sealcoated pavement and UVA as much as a month after sealcoat application has the potential to cause genotoxicity and impair DNA repair capacity.

Keywords : Genotoxicity DNA repair activity Polycyclic aromatic hydrocarbon N-heterocycle Coal tar Urban runoff

Document type :

Journal articles

Domain :

Environmental Sciences / Biodiversity and Ecology

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https://hal-sde.archives-ouvertes.fr/hal-01292436
Contributor : Nathalie Lyvet <>
Submitted on : Wednesday, March 23, 2016 - 10:28:42 AM
Last modification on : Thursday, April 19, 2018 - 2:28:02 PM

Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1fish liver cell line.

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Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1fish liver cell line.

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