Experimental assessment of the bioconcentration of 15N-tamoxifen in Pseudokirchneriella subcapitata

Frédéric Orias 1 Laurent Simon 2 Yves Perrodin 1
1 Impacts des Polluants sur les Écosystèmes
LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés
2 Écologie, Évolution, Écosystemes Souterrains
IUF - Institut Universitaire de France, LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés
Abstract : Nowadays, pharmaceutical compounds (PC) are ubiquitous in aquatic ecosystems. In addition to direct ecotoxicity, the bioconcentration of PC in organisms is a phenomenon which could have an impact on the whole ecosystem. In order to study this phenomenon, we exposed unicellular algae (Pseudokirchneri- ella subcapitata) to 15N-tamoxifen, an anticancer drug labelled with a stable nitrogen isotope used as a tracer. By measuring 15N enrichment over time, we were able to measure the increase of tamoxifen con- tent in algae. This enrichment was measured by an elemental analyser coupled with an isotopic ratio mass spectrometer (EA–IRMS). Algal cells were exposed for 7 d to 3 concentrations of tamoxifen: 1, 10 and 100 lg L1. Our result shows a high bioconcentration in algae from the first minutes of contact. The highest bioconcentration factor measured is around 26500. We also observe that bioconcentration is not linked to the exposure concentration. This study is the first to use stable isotopes in order to monitor PCs in aquatic organisms such as algae. The use of stable isotopes in ecotoxicology offers interesting perspectives in the field of contaminant transfer in organisms and along the trophic web.
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Article dans une revue
Chemosphere, Elsevier, 2015, 122, pp.251-256. 〈10.1016/j.chemosphere.2014.11.070〉
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Soumis le : lundi 2 mars 2015 - 14:02:40
Dernière modification le : mardi 16 janvier 2018 - 16:12:47

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Frédéric Orias, Laurent Simon, Yves Perrodin. Experimental assessment of the bioconcentration of 15N-tamoxifen in Pseudokirchneriella subcapitata. Chemosphere, Elsevier, 2015, 122, pp.251-256. 〈10.1016/j.chemosphere.2014.11.070〉. 〈hal-01121690〉

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