Evidence for biological denitrification inhibition (BDI) by plant secondary metabolites

Abstract : Previous studies on the effect of secondary metabolites on the functioning of rhizosphere microbial communities have often focused on aspects of the nitrogen (N) cycle but have over- looked biological denitrification inhibition (BDI), which can affect plant N-nutrition. Here, we investigated the BDI by the compounds of Fallopia spp., an invasive weed shown to be associ- ated with a low potential denitrification of the soil.Fallopia spp. extracts were characterized by chromatographic analysis and were used to test the BDI effects on the metabolic and respiratory activities of denitrifying bacteria, under aerobic and anaerobic (denitrification) conditions. The BDI of Fallopia spp. extracts was tested on a complex soil community by measuring denitrification enzyme activity (DEA), substrate induced respiration (SIR), as well as abundances of denitrifiers and total bacteria.In 15 strains of denitrifying bacteria, extracts led to a greater BDI (92%) than respiration inhibition (50%). Anaerobic metabolic activity reduction was correlated with catechin concen- trations and the BDI was dose dependent. In soil, extracts reduced the DEA/SIR ratio without affecting the denitrifiers: total bacteria ratio.We show that secondary metabolite(s) from Fallopia spp. inhibit denitrification. This pro- vides new insight into plant–soil interactions and improves our understanding of a plant’s abil- ity to shape microbial soil functioning.
Type de document :
Article dans une revue
New Phytologist, Wiley, 2014, 204, pp.620-630. 〈10.1111/nph.12944〉
Liste complète des métadonnées

https://hal-sde.archives-ouvertes.fr/hal-01085302
Contributeur : Nathalie Lyvet <>
Soumis le : vendredi 21 novembre 2014 - 10:42:57
Dernière modification le : mardi 16 janvier 2018 - 16:12:15

Identifiants

Collections

Citation

Clément Bardon, Florence Piola, Floriant Bellvert, Feth El Zahar Haichar, Gilles Comte, et al.. Evidence for biological denitrification inhibition (BDI) by plant secondary metabolites. New Phytologist, Wiley, 2014, 204, pp.620-630. 〈10.1111/nph.12944〉. 〈hal-01085302〉

Partager

Métriques

Consultations de la notice

79