HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

A new ultradian rhythm in mammalian cell dry mass observed by holography

Abstract : Abstract We have discovered a new 4 h ultradian rhythm that occurs during the interphase of the cell cycle in a wide range of individual mammalian cells, including both primary and transformed cells. The rhythm was detected by holographic lens-free microscopy that follows the histories of the dry mass of thousands of single live cells simultaneously, each at a resolution of five minutes. It was vital that the rhythm was observed in inherently heterogeneous cell populations, thus eliminating synchronization and labeling bias. The rhythm is independent of circadian rhythm, and is temperature-compensated. We show that the amplitude of the fundamental frequency provides a way to quantify the effects of, chemical reagents on cells, thus shedding light on its mechanism. The rhythm is suppressed by proteostasis disruptors and is detected only in proliferating cells, suggesting that it represents a massive degradation and re-synthesis of protein every 4 h in growing cells.
Document type :
Journal articles
Complete list of metadata

Contributor : Lamya Ghenim Connect in order to contact the contributor
Submitted on : Friday, November 26, 2021 - 9:11:11 AM
Last modification on : Friday, April 1, 2022 - 3:52:08 AM
Long-term archiving on: : Sunday, February 27, 2022 - 6:15:02 PM


s41598-020-79661-9 (7)_SR_Ultr...
Publisher files allowed on an open archive



Lamya Ghenim, Cédric Allier, Patricia Obeid, Lionel Hervé, Jean-Yves Fortin, et al.. A new ultradian rhythm in mammalian cell dry mass observed by holography. Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-020-79661-9⟩. ⟨hal-03450438⟩



Record views


Files downloads