Native chromatin immunoprecipitation.

Céline Cosseau (1) , Christoph Grunau (1)

1 2EI - Ecologie et évolution des interactions [2011-2014]

Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin immunoprecipitation. We describe here a protocol that was specifically optimized for low cell numbers.

Domaines: Biodiversité et Ecologie

Céline Cosseau, Christoph Grunau. Native chromatin immunoprecipitation.. Methods in Molecular Biology, 2011, 791, pp.195-212. ⟨10.1007/978-1-61779-316-5_15⟩. ⟨halsde-00629480⟩