Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages

S. Beltran (1) , Richard Galinier (1) , Jean-François Allienne (1) , Jérôme Boissier (1)

1 BETM - Biologie et écologie tropicale et méditerranéenne [2007-2010]

Schistosomes represent a unique animal model for comparative analyses of monogamy. Indeed, schistosomes are classified at the lowest taxonomical level of monogamous species and lack complex social interactions, which could alter our understanding of their unusual mating system. Elements discussed here include the fact that monogamy in schistosomes could be an ancestral state between hermaphroditism and polygyny or polygynandry and the occurrence of mate changes. In addition, hypotheses are proposed to explain monogamy in schistosomes (e.g. female dispersion, the need for paternal care, oviposition site limitation or aggressiveness, and mate guarding). We also propose future experimental and analytical approaches to improve our understanding of the schistosomes' mating system.

Mots clés: Schistosoma mansoni; DNA extraction; microsatellites

Domaines: Biodiversité et Ecologie

S. Beltran, Richard Galinier, Jean-François Allienne, Jérôme Boissier. Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages. Memórias do Instituto Oswaldo Cruz, 2008, 103 (5), pp.501-503. ⟨halsde-00344405⟩