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| HAL : halsde-00180951, version 1 |
| DOI : 10.1055/s-2007-965258 |
| Fiche détaillée |  Récupérer au format |
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| Plant Biology / Plant biol (Stuttg) (2007) ISSN 1435-8603 |
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| Truncated Hemoglobins in Actinorhizal Nodules of Datisca glomerata |
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| K. Pawlowski 1, 2, 3K. R. Jacobsen 4 |
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| (2007) |
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| Three types of hemoglobins exist in higher plants, symbiotic, non-symbiotic, and truncated hemoglobins. Symbiotic (class II) hemoglobins play a role in oxygen supply to intracellular nitrogen-fixing symbionts in legume root nodules, and in one case (Parasponia sp.), a non-symbiotic (class I) hemoglobin has been recruited for this function. Here we report the induction of a host gene, dgtrHb1, encoding a truncated hemoglobin in Frankia-induced nodules of the actinorhizal plant Datisca glomerata. Induction takes place specifically in cells infected by the microsymbiont, prior to the onset of bacterial nitrogen fixation. A bacterial gene (Frankia trHbO) encoding a truncated hemoglobin with O2-binding kinetics suitable for the facilitation of O2 diffusion (Tjepkema et al., 2002) is also expressed in symbiosis. Nodule oximetry confirms the presence of a molecule that binds oxygen reversibly in D. glomerata nodules, but indicates a low overall hemoglobin concentration suggesting a local function. Frankia TrHbO is likely to be responsible for this activity. The function of the D. glomerata truncated hemoglobin is unknown; a possible role in nitric oxide detoxification is suggested. |
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| 1 : | Albrecht von Haller Institute for Plant Sciences Department of Plant Biochemistry |
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Göttingen University |
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| 2 : | Department of Molecular Biology |
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wageningen University |
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| 3 : | Department of Botany |
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Stockholm University |
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| 4 : | department of plant sciences |
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University of California, Davis |
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| 5 : | Ecologie microbienne (EM) |
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CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon |
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| 6 : | Ecology, Evolution, and Behavior, University of Minnesota, |
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University of Minnesota |
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| 7 : | Department of Plant Biology |
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Cornell University |
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| 8 : | Department of Biological Sciences |
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University of Maine |
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| 9 : | Molecular Phytopathology |
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Göttingen University |
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| 10 : | Department of Plant Pathology |
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University of Wisconsin-Madison |
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| domaine | : | Sciences de l'environnement/Biodiversité et Ecologie Sciences du Vivant/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique |
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| Nitrogen-fixing root nodules – leghemoglobin – hemoglobin – nitric oxide – glb3 – trHb. |
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| halsde-00180951, version 1 | |
| http://hal-sde.archives-ouvertes.fr/halsde-00180951 | |
| oai:hal-sde.archives-ouvertes.fr:halsde-00180951 | |
| Contributeur : Christine Delorme | |
| Soumis le : Lundi 22 Octobre 2007, 14:59:53 | |
| Dernière modification le : Lundi 22 Octobre 2007, 16:12:29 | |
