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| HAL: halsde-00155880, version 1 |
| DOI: 10.1111/j.1574-6968.2006.00564.x |
| Detailed view |  Export this paper |
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| FEMS Microbiology Letters / FEMS Microbiological Letters 268 (2007) 67-72 |
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| Green fuorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum |
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| David Rekangalt 1Marie-Christine Verner 1 |
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| (2007) |
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| The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant–fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum. |
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| 1: | Ecologie microbienne (EM) |
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CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon |
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| 2: | Institute of Forest Botany, Molecular Wood Biotechnology |
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Georg-August-University |
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| 3: | Institute of Microbiology |
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ETH Zuric |
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| subject | : | Environmental Sciences/Biodiversité et Ecologie Environmental Sciences/Biodiversité et Ecologie/Ecologie Microbienne Life Sciences/Microbiology and Parasitology/Mycology |
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| reporter gene – green fluorescent protein – ectomycorrhiza – Hebeloma cylindrosporum. |
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| halsde-00155880, version 1 | |
| http://hal-sde.archives-ouvertes.fr/halsde-00155880 | |
| oai:hal-sde.archives-ouvertes.fr:halsde-00155880 | |
| From: Christine Delorme | |
| Submitted on: Tuesday, 19 June 2007 14:12:02 | |
| Updated on: Tuesday, 19 June 2007 14:48:43 | |
