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| HAL : hal-00663883, version 1 |
| DOI : 10.1111/j.1742-4658.2009.06982.x |
| Fiche détaillée |  Récupérer au format |
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| FEBS Journal 9 (2009) 2566-2573 |
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| In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
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| Caroline Fabioux 1, 2Charlotte Corporeau 2, 3 |
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| (2009) |
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| This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. |
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| 1 : | Laboratoire des Sciences de l'Environnement Marin (LEMAR) |
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Institut Universitaire Européen de la Mer (IUEM) – Université de Bretagne Occidentale - Brest – CNRS : UMR6539 – Institut de Recherche pour le Développement |
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| 2 : | Physiologie et Ecophysiologie des Mollusques Marins (PE2M) |
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Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
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| 3 : | Physiologie et Ecophysiologie des Mollusques Marins (PE2M) |
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CNRS : UMR100 – Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
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| Domaine | : | Sciences de l'environnement |
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| Crassostrea gigas – germline – marine bivalve – RNAi – vasa |
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| hal-00663883, version 1 | |
| http://hal.univ-brest.fr/hal-00663883 | |
| oai:hal.univ-brest.fr:hal-00663883 | |
| Contributeur : Catherine Bertignac | |
| Soumis le : Vendredi 27 Janvier 2012, 17:11:25 | |
| Dernière modification le : Vendredi 27 Janvier 2012, 17:11:25 | |
